温州蜜柑果皮提取物总酚·总黄酮含量测定及其抗氧化活性研究

目的]研究温州蜜柑果皮不同溶剂提取物总酚、总黄酮含量及其抗氧化活性,筛选最佳提取试剂。方法]依次用甲醇、乙醇、丙酮、乙酸乙酯及蒸馏水对温洲蜜柑果皮进行提取,检测不同溶剂提取物体外抗氧化活性,并对不同溶剂提取物总酚、总黄酮含量进行测定。结果]温州蜜柑不同溶剂提取物均具有一定的抗氧化活性,其中甲醇、丙酮提取物的抗氧化活性较强,APC指数位居前2位。不同溶剂提取物的抗氧化活性与其总酚、总黄酮的含量呈极显著正相关关系,相关系数r分别为0.905**、0.583**、0.948**、0.849**、0.760**、0.955**(P0.01)。结论]对温州蜜柑不同溶剂提取物总酚、总黄酮含量测定及其抗氧化活性研究确定甲醇为其最优提取试剂。

Detection of Total Phe nols and Total Flavonoids Content in Extracts of Citrus unshiu Peel and Its Antioxidant Activities

Objective] The aim was to compare and study content of total phenols and total flavonoids in Citrus unshiu peel and antioxidant ac-tivity by different solvents,to screen out optimal extraction reagent. Method] Methanol,ethanol,acetone,ethyl acetate and distilled water was used to get extracts from Citrus unshiu peel.The antioxidant activity was detected.The content of total phenol,total flavonoids in different solvents was determined.Result] The different solvents from Cit rus unshiu all had some antioxidant activity.The extracts from methanol and acetone had more stronger antioxidant activity than others,APC index was in top 2.The antioxidant activity had extremely significant positive correlation with the content of total flavonois,total phenols(r=0.905**,0.583**,0.948**,0.849**,0.760**,0.955**(P<0.01)).Conclusion] The ex-tracts of methanol of Citrus unshiu show the best in antioxidant activities and contents of total flavonoids and phenols from different solvent extracts of Ctirus unshiu.