南方稻区国家水稻区域试验品种的微卫星标记分析

利用前期研究筛选的12个微卫星标记（SSR）首选标记对2005年南方稻区国家水稻区域试验199个水稻品种进行了DNA指纹鉴定，构建了199个水稻品种×12个首选标记的南方稻区国家水稻区域试验品种DNA指纹库。在首选标记座位上，常规稻和杂交稻的主导等位基因基本相同，籼稻和粳稻的主导等位基因差异较大；常规稻品种的纯合度高，多数杂交稻品种的杂合度较高、杂合座位数呈正态分布。比较相同母本的系列杂交稻组合，表明主导不育系系列组合具有较高的组内品种间母本一致性。此外，对南方稻区国家水稻区域试验品种特异性鉴定、DNA指纹库的扩充和鉴定标记数的确定进行了讨论。关键词

Microsatellite Marker-Based Analysis of Rice Varieties in National Regional Yield Trial of Southern China

A total of 199 rice varieties in the national regional yield trial of southern China in 2005 were assayed with a preferred set of 12 microsatellite markers(SSR). A DNA fingerprint database containing genotypic data of the 199 rice varieties at the 12 marker loci was constructed. It was found that the dominant alleles in conventional rice varieties were the same as in hybrid rice at most of the marker loci, but they differed greatly from indica to japonica rice. Conventional rice was of high homozygosity and most hybrid rice was of high heterozygosity. The variation in number of heterozygous loci was evident for hybrid rice with a normal distribution. On comparison among hybrid rice combinations derived from the same cytoplasmic male sterile(CMS) line, general consistency of the maternal allele of major CMS lines was observed at the SSR loci. Moreover, the distinctness assay of rice variety in the national regional yield trial of southern China, and the expansion of DNA fingerprint database and the number of SSR markers for a feasible test were discussed.