巴什拜羊群体双肌基因基因型检测

【目的】通过对巴什拜羊SNPCLPG的分子检测,期望发现CLPG基因型种羊,为今后选育肉用性状更优异的新品系奠定基础。【方法】绵羊的双肌臀(Callipyge,CLPG)基因表型为后臀肌肉增大近30%,是由位于绵羊18号染色体GTL2基因上游32.8 kb处1个A→G的单核苷酸多态性(SNP)标记(命名SNPCLPG)突变所产生的。为此,选择设计合成了1对引物,采用聚合酶链式反应一单链构象多态性(PCR—SSCP)方法,以巴什拜羊作为研究样本,进行了SNPCLPG位点的PCR检测。【结果】获得了预期的扩增片段,并检测到了基因多态性,但未检测到CLPG基因型特征的SNPCLPG突变(A→G)。【结论】被检测的巴什拜羊群体中该区域没有发现A→G的单核苷酸多态性标记突变。

Detection of the Callipyge Genotype of Bashbay Sheep Population

【Objective】Though the molecular testing of the SNPCLPG of Bashbay Sheep,the paper expected to find CLPG genotype sheep in order to lay a good foundation for future selective breeding of new species with better meat traits.【Method】The phenotype of the Callipyge(CLPG) gene of sheep is an increase of nearly 30% of muscles in its back hip,and it is generated by a mutation from a A→G single nucleotide polymorphism(SNP) marker(named as SNPCLPG)that is located in the 32.8 kb point of the upstream of the GTL 2 gene of the No 18 chromosome of the sheep.Therefore,a pair of primers were selected,designed and synthesized;using Bashbay sheep as research material,and adopting polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) method,the PCR testing of the SNPCLPG locus was conducted.【Result】Expected amplified fragments were obtianed,and polymorphism was detected,but the SNPCLPG mutation(A→G) of the CLPG genotype was not detected.【Conclusion】There is no A→G mutation of single nucleotide polymorphism marker detected in the tested Bashbay sheep population in the region.