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9株芽孢杆菌的初步分离鉴定与拮抗性试验
引用本文:来航线,杨保伟,邱学礼.9株芽孢杆菌的初步分离鉴定与拮抗性试验[J].西北农林科技大学学报(社会科学版),2004,32(7):93-96.
作者姓名:来航线  杨保伟  邱学礼
作者单位:西北农林科技大学,资源环境学院,陕西,杨凌,712100
摘    要:通过对采集样品中细菌的分离与纯化,获得9株芽孢杆菌,经纯化培养后观察其个体和群体形态特征,并进行了12项生理生化特征鉴定。结果表明,B10为地衣芽孢杆菌,B13为巨大芽孢杆菌,B12、B14为短小芽孢杆菌,B15为苏芸金芽孢杆菌,B16为蕈状芽孢杆菌,B11,B17,B18为蜡样芽孢杆菌。通过对8株植物病原菌和3株动物病原菌的拮抗试验,初步确定B13,B16,B17,B18的抗菌谱较广,其中B10,B15对棉花枯萎菌,B13,B17对辣椒疫霉菌,B11对西瓜枯萎菌,B10,B17,B18对3株动物病原菌有较好的拮抗能力。

关 键 词:芽孢杆菌(Bacillus)  分离鉴定  拮抗试验
文章编号:1671-9387(2004)07-0093-04
收稿时间:2003/6/10 0:00:00
修稿时间:2003年6月10日

Isolation indentification and antibiotic experiment of nine strains of Bacillus cereus.B.C
LAI Hang-xian,YANY Bao-wei,QIU Xue-li,XUE Quan-hong.Isolation indentification and antibiotic experiment of nine strains of Bacillus cereus.B.C[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(7):93-96.
Authors:LAI Hang-xian  YANY Bao-wei  QIU Xue-li  XUE Quan-hong
Institution:(College of Resource and Environmental Science,Northwest Sci-Tech University of Agriculture and Foresty,Yangling,Shaanxi 712100,China)
Abstract:Nine strains of Bacillus cereus.B.C were isolated from several samples and identification of the nine strains'characters of individual and integral morphology was made,and the identification is physiology and biochemistry experiments with Bacillus cereus.B.C classification standards.The results show:B10 was Bac.licheniformis,B13 was Bacillus megaterium,B12 and B14 were Brevibacilli breris.B.r.B15 was Bacillus thuringiensis,B.t,B16 was B.mycoides Flugge,B11,B17 and B18 were Bacilli cereus.B.The results of antibiotic experiments indicated nine strains of Bacillus cereus.B.C had antibiolosis to three strain animal pathogens and eight strain plant pathogens.
Keywords:Bacillus  isolation and identification  antibiolotic experiment
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