|
|||||
|
|
Cultivation, LD(50) determination and experimental model of Streptococcus suis serotype 2 strain HA9801 |
|
| Authors: | Zhao Zhanzhong Wang Jian Liu Peihong Zhang Suhua Gong Jianpei Huang Xiqin Li Bin Xue Feiqun |
| Institution: | a Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, China b Shanghai Animal Disease Control Center, Shanghai 201103, China c Department of Research and Development, Shanghai Hile Biopharmaceutical Co., Ltd., Shanghai 201400, China |
| Abstract: | The effects of nutritional components and submerged culture conditions on colony-forming unit (CFU) counts by Streptococcus suis serotype 2 strain HA9801 in flask culture was investigated, and the optimal medium and cultivation conditions was confirmed by using a 50 l bioreactor. The LD50 values of HA9801 in pigs before and after fermentation were 1.8 × 107 CFU, which indicated that the virulence of HA9801 was very stable in the fermentation process. In addition, an experimental model that closely mimics naturally occurring disease in conventional pigs was established. |
| Keywords: | Streptococcus suis serotype 2 HA9801 Optimization LD50 Experimental model |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |