Cytokine expression in colostrum-deprived pigs immunized and challenged with Haemophilus parasuis |
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Authors: | AJ Martín de la Fuente F Tejerina S Martínez Martínez |
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Institution: | a Department of Animal Health, Section of Microbiology and Immunology, University of León, 24007-León, Spain b Department of Veterinary Medicine, Surgery and Anatomy, Section of Reproduction, Faculty of Veterinary Medicine, University of León, 24007-León, Spain |
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Abstract: | The expression of several cytokines in spleen, pharyngeal lymph nodes, lung and brain after different immunization procedures and a challenge with 5 × 109 CFU of Haemophilus parasuis was compared. Five groups of colostrum-deprived pigs were used: vaccinated with (I) a bacterin, (II) an outer-membrane-protein-vaccine, (III) a recombinant transferring-binding protein B, (IV) exposed to a total dose of 105 CFU, and (V) not previously immunized. All pigs in groups III and V died, while all animals in group I, most of group IV and half of group II survived until the end of the experiment. IL-1α was found in significantly higher levels (p < 0.05) in spleen, lymph nodes and brain of dead pigs, which could be explained by the major severity of lesions in these animals. However, IL-4, IL-10, TNF-α and IFN-γ were expressed in significantly higher levels by survivors (for all the four cytokines in lymph nodes; for IL-4, IL-10 and TNF-α in spleen; for IL-4, TNF-α and IFN-γ in lung, and only for TNF-α in brain), thus suggesting a role of these four cytokines in the adaptive response, which might contribute to protection against H. parasuis infection. |
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Keywords: | Haemophilus parasuis Glä sser&rsquo s disease Pig Cytokines Vaccine |
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