A proteomic reference map for pig serum proteins as a prerequisite for diagnostic applications |
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Authors: | Miller Ingrid Wait Robin Sipos Wolfgang Gemeiner Manfred |
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Institution: | a Institute of Medical Chemistry, Department of Natural Sciences, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria b Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science, Technology and Medicine, 1, Aspenlea Road, Hammersmith, London W6 8LH, UK c Clinic for Swine, Clinical Department for Farm Animals and Herd Management, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria |
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Abstract: | A reference protein map for pig serum was set up using two-dimensional electrophoresis (2-DE). Thirty-nine protein chains or spots deriving from 26 different proteins were identified by immunological and mass spectrometric methods. Thus, the positions of most medium to higher abundance serum proteins could be determined on the 2-DE gels. The plasma protein fibrinogen was also included in our study. The overall pig protein pattern differs in some respect to serum/plasma maps of other mammalian species, e.g. in levels and properties of single proteins such as haptoglobin or IgM or in species-specific proteins like pig major acute phase protein. Serum protein maps are a useful tool to get an overview on expressed proteins, and to monitor changes in concentration as well as isotype distribution of the identified proteins. As a consequence, more detailed knowledge on protein pattern changes may give deeper insights into the metabolic development of some pathologic conditions and may lead to putative biomarkers for further investigation. Selected examples for protein pattern changes in pigs infected by a viral (porcine circovirus type 2) and a bacterial (Actinobacillus pleuropneumoniae) pathogen illustrate the usefulness of the method. |
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Keywords: | 1D one-dimensional 2-DE two-dimensional electrophoresis App Actinobacillus pleuropneumoniae Ig immunoglobulin IPG immobilized pH-gradient MAP major acute phase protein PCV2 porcine circovirus type 2 pI isoelectric point PMWS postweaning multisystemic wasting syndrome |
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