Identification of wheat–Thinopyrum intermedium 2Ai-2 ditelosomic addition and substitution lines with resistance to barley yellow dwarf virus |
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Authors: | Z. S. Lin D. H. Huang L. P. Du X. G. Ye Z. Y. Xin |
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Affiliation: | Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China;;Corresponding author, E-mail: |
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Abstract: | Among the regenerated plants derived from immature hybrid embryos of wheat–Thinopyrum intermedium disomic addition line Z6 × common wheat variety ‘Zhong8601’, a plant with a telocentric chromosome and barley yellow dwarf virus (BYDV) resistance was obtained. The telocentric chromosome paired with an entire Thinopyrum chromosome to form a heteromorphic bivalent at meiotic metaphase I. Genomic in situ hybridization showed that the telosome originated from Th. intermedium. Two ditelosomic additions and one disomic substitution were identified among the offspring of the plant. Two random amplified polymorphic DNA molecular markers were identified among 150 random primers used to detect the different arms of the alien chromosome. These might be useful for developing translocation lines with BYDV resistance. |
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Keywords: | Triticum aestivum Thinopyrum intermedium barley yellow dwarf virus chromosome substitution ditelosomic addition line genomic in situ hybridization random amplified polymorphic DNA |
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