东乡普通野生稻根际微生物群落特征的研究

目的]研究东乡普通野生稻（Oryza rufipogon Griff.）根际微生物群落特征。方法]采用氯仿熏蒸浸提法测定根系微生物生物量碳、氮;采用平板稀释培养法测定群落组成;采用最大或然计数法测定微生物生理群。结果]东乡野生稻根际微生物生物量碳、氮平均值分别为83.02和16.98mg/kg（土）,较普通栽培稻低;根际可培养微生物中细菌〉放线菌〉真菌;氮素生理群中氨化细菌〉好氧固氮菌〉反硝化细菌〉嫌气性固氮菌〉硝化细菌〉亚硝化细菌。结论]东乡野生稻根际微生物群落组成与普通栽培稻相比有差异。

Studies on the Microbial Community in the Rhizosphere Soil of Dongxiang Wild Rice

Objective] The aim was to study the characteristics of microbial community in the rhizosphere soil of Dongxiang wild rice(Oryza rufipogon Griff.).Method] The microbial biomass carbon and nitrogen was estimated by the chloroform fumigation extraction method;the microbial community composition and Nitrogen cycling microbial functional groups were investigated by the Dilution plate culture method and the most probable number methods respectively.Result] The microbial biomass carbon and nitrogen,in rhizosphere soil of Dongxiang Wild Rice was 83.02±18.23 mg/kg soil and 16.98±2.54 mg/kg soil,which was lower than that of ordinary cultivated rice;The relationship between the number of culturable microbial groups was bacteria>actinomycetes>fungi,and the Nitrogen cycling microbial physiological groups was as the following:ammonifying bacteria>aerobic azotobacteria>denitrobacteria>anaerobic azotobacteria>nitrobacteria>nitrosobacteria.Conclusion] The microbial community in the rhizosphere soil of Dongxiang Wild Rice was different from that of the ordinary cultivated rice.