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湖北玉米粗缩病的检测与鉴定
引用本文:章松柏,李红,张求东,杨俊杰,张植敏,张长青,吴祖建.湖北玉米粗缩病的检测与鉴定[J].玉米科学,2013,21(3):136-139.
作者姓名:章松柏  李红  张求东  杨俊杰  张植敏  张长青  吴祖建
作者单位:长江大学农学院, 湖北 荆州 434025;福建农林大学植物病毒所, 福建 福州 350002;湖北省植物保护总站, 武汉 430070;湖北省植物保护总站, 武汉 430070;湖北省植物保护总站, 武汉 430070;湖北省长阳县植保站, 湖北 长阳 443500;长江大学农学院, 湖北 荆州 434025;福建农林大学植物病毒所, 福建 福州 350002
基金项目:农业部公益性行业专项子项(201203032-13)、长江大学校基金项目(2006Z2070)
摘    要:田间调查发现,湖北省长阳县玉米粗缩病发生普遍,白背飞虱大量分布,未见灰飞虱存在。通过症状观察、病毒dsRNA基因组电泳图谱分析、RT-PCR、序列分析等鉴定方法,对湖北长阳玉米粗缩病进行病原鉴定。结果表明,湖北长阳发生的玉米粗缩病是由新的水稻斐济病毒暂定种——南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)引起。

关 键 词:玉米粗缩病  南方水稻黑条矮缩病毒  dsRNA  白背飞虱
收稿时间:2012/8/12 0:00:00

Detection and Identification of Maize Rough Dwarf Disease in Hubei
ZHANG Song-bai,LI Hong,ZHANG Qiu-dong,YANG Jun-jie,ZHANG Zhi-min,ZHANG Chang-qing and WU Zu-jian.Detection and Identification of Maize Rough Dwarf Disease in Hubei[J].Journal of Maize Sciences,2013,21(3):136-139.
Authors:ZHANG Song-bai  LI Hong  ZHANG Qiu-dong  YANG Jun-jie  ZHANG Zhi-min  ZHANG Chang-qing and WU Zu-jian
Institution:College of Agriculture, Yangtze University, Jingzhou 434025;Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Hubei Plant Protection Station, Wuhan 430070;Hubei Plant Protection Station, Wuhan 430070;Hubei Plant Protection Station, Wuhan 430070;Plant Protecting Station of Changyang County, Changyang 443500;College of Agriculture, Yangtze University, Jingzhou 434025;Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract:Field investigations were conducted on the viral diseases in Changyang county, Hubei province. Maize rough dwarf disease was the main virus disease with vast white-backed planthoppers but few small brown planthoppers. By symptom observation, dsRNA profiling, RT-PCR and sequence analysis, the pathogen of the maize rough dwarf disease in Changyang county was identified as the proposed novel Fijivirus species, the name is Southern rice black-streaked dwarf virus(SRBSDV).
Keywords:Maize rough dwarf disease  Southern rice black-streaked dwarf virus  dsRNA  Sogatella furcifera
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