基于EST-SSR毛细管电泳荧光标记技术分析广东2个历史名茶群体遗传多样性

利用EST-SSR毛细管电泳荧光标记技术对罗坑和马图2个广东历史名茶茶树群体的共52份种质资源进行研究。结果表明:27对引物共检测到204个位点,平均每对引物检测7.56个位点,引物检测到的基因型数为11.22个;观测等位基因数为7.56,有效等位基因数为3.42;观测杂合度和期望杂合度平均值分别为0.55和0.66;多态性信息指数(PIC)平均值为0.61;反应遗传多样性的Shannon’s遗传信息指数为1.40。2个茶树群体的基因流Nm平均值为3.19,表明2个群体间在过去的某个时间可能有过基因交流,群体间遗传分化系数(Fst)的平均值为0.072 7;2个亲体的遗传多样性水平相近。依据Evanno统计模型分析可将52份茶树种质分为3个亚群,分别包含18、24、9份茶树种质资源,MT4种质没有明确的亚类特性。 

Genetic Diversity and Relationship of Two Historical Famous Camellia Sinensis Groups in Gugangdong by Capillary Electrophoresis Detcection with Fluorescent EST-SSR Marker

EST -SSR capillary electrophoresis fluorescence maker technique was used to analyze the genetic polymorphism and genetic relationship of 52 tea plant germplasm resources from two historical famous Camellia Sinensis groups of Luokeng and MaTu in Guangdong. In total, 204 alleles were detected by 27 EST-SSR primers,      with an average of 7.56 locus per primer, and the genotypes was 11.22.  The  numbers  of  observed  alleles  and  effective alleles was 7.56 and 3.42. The observed heterozygosity ( Ho) and expected heterozygosity （He） was 0.55 and 0.66, respectively. The average of polymorphism information content (PIC) was 0.61, and the Shannon index of    the 52 germplasms was 1.40. The Nm ( gene flow)  of  the  two  groups  was  3.19,  showing  gene  exchange  might occur in the past time between the two populations. And Fst was 0.072 7. The  genetic  diversity  of  the  two  groups  was similar. The 52 tea plant germplasms were divided into three subgroups containing  18,  24,  9  tea  plant  germplasms resources, respectively, based on statistical model of Evanno egc., and the germplasm of MT4 did not belong to any subgroups.