红掌细菌性疫病病原菌遗传多样性的 RAPD 和 Rep-PCR分析

由地毯草黄单胞菌花叶万年青致病变种引起的细菌性疫病是红掌生产中最具破坏性的病害。本研究对来自全国 8 个省市主产区的 93 个红掌细菌性疫病菌株,采用 RAPD 和 Rep-PCR（ERIC、BOX、REP）分子标记进行了遗传多样性分析。筛选出的 13 个 RAPD 引物共扩增出 131 个清晰、重复性好的条带,而 Rep-PCR 共扩增出65 个条带,所有位点多态性比率为 100%。基于 RAPD 和 Rep-PCR 结果计算的群体遗传距离显著相关,综合 2 种标记类型的分型结果进行 UPGMA 聚类分析,表明我国红掌细菌性疫病具有高度的遗传多样性,在遗传相似性系数范围 0.519 1~0.984 7,在 0.63 水平时可以将所有菌株划分为 3 个组群,分别包括 58、28、7 个个体,病菌群体的遗传多样性与其寄主品种、地理来源没有明显相关性。本研究为红掌细菌性疫病监测和抗病育种提供了科学依据。

Genetic Diversity Among Xanthomonas axonopodis pv. dieffenbachiaeIsolates from Anthurium Assessed by Combining RAPD and RepPCR

Bacterial blight is the most devastating disease of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad). In this study, ninety-three isolates of Xad from different geographical locations in China were analyzed for genetic diversity using RAPD and Rep-PCR (ERIC, BOX, REP). A total of 131 clear and reproducible bands were amplified from 13 selected RAPD primers, and 65 bands were amplified by Rep-PCR. The polymorphism rate of all sites was 100%. Based on the fingerprint data, cluster analysis were performed by using the unweighted pair group method, arithmetic average (UPGMA) program, which showed a high genetical diversity among the isolates, with genetic similarity varied from 0.519 1 to 0.984 7. At a similarity of 0.63, all isolates were grouped into three genetic groups (Ⅰ-Ⅲ), including 58, 28, 7 isolates, respectively. Moreover, no significant correlation between genetic diversity and geographical origin of the isolates. This present data would provide a scientific support for blight monitoring and resistance breeding in anthurium.