兰科植物FNR基因的密码子偏好性分析

为揭示兰科植物FNR基因的特性和密码子偏好性,采用DNAMAN、CodonW和SPSS软件及 EMBOSS、SWISS-MODEL在线网站对17种兰科植物的FNR基因序列、氨基酸序列、同源区域及蛋白质三维结构进行分析。结果表明：兰科植物FNR基因普遍具有在A/T(U)与G/C之间较弱的密码子偏好性;密码子的末位在A和T(U)之间存在显著的T(U)偏好性,在C和G之间存在显著的C偏好性;NADP结合域比非结合域有较小的密码子偏好性;基于CDS和氨基酸聚类结果比基于RSCU聚类更接近于植物的进化分类;自然选择的作用是导致兰科植物FNR基因的密码子使用偏好性的主要成因。

Codon Usage Bias of Ferredoxin-NADP+Oxidoreductase (FNR) in Orchidaceae

For a good understanding of the codon usage bias of ferredoxin-NADP+oxidoreductase (FNR) in Orchidaceae, the gene sequence, amino acid sequence, homologous region and three-dimensional protein structure of 17 FNR genes in Orchidaceae were analyzed by DNAMAN, CodonW, SPSS softwares, and EMBOSS, SWISS-MODEL online programs. The results showed that there was a low-level codon usage bias in FNR genes between A/T(U) and G/C; there existed a significant T(U) bias between A and T(U) and a significant C bias between C and G in the codons; NADP binding domain showed less codon bias than that of the non-binding domain; the cluster tree based on coding sequence (CDS) as well as amino acid sequences matched better with the plant evolution classification; the codon usage bias of FNR was mainly influenced by the natural selection in Orchidaceae.