瓜实蝇性别决定基因tra——2的克隆及表达分析

本研究对瓜实蝇Bactrocera cucurbitae Coqillet的性别决定基因tra- 2的eDNA序列进行了克隆和分析。根据转录组数据库及序列同源性分析,分别克隆并获得瓜实蝇雌雄成虫tra- 2的基因片段,利用生物信息软件对所得序列进行分析;采用qPCR技术,研究该基因在瓜实蝇不同发育阶段的表达情况。研究结果表明,瓜实蝇ra-2基因的eDNA序列共1 450 bp,无性别特异性,含753 bp的开放阅读框,共编码250个氨基酸。 该基因在瓜实蝇不同发育阶段均有不同的表达量,瓜实蝇tra-2基因具有母体遗传表达特性,在胚胎期的表达量较高,雌成虫表达量显著高于雄成虫。

Cloning,Expression Analysis of Sex- determining Genes tra- -2 in MelonFly,Bactrocera cucurbitae Coquillett Diptera:Tephritidae#br#

In this paper, the sex determination gene tra-2 eDNA sequence of B. cucurbitae Coquillett was cloned and analyzed. According to the transcriptome database and sequence homology analysis, the male and female tra-2 gene fragments of the B. cucurbitae were cloned and analyzed by bioinformatics software. The qPCR technique was used to study the ffcts of diferent development stage of the expression of the situation. The results showed that the eDNA sequence of tra-2 gene of B. cucurbitae was 1 450 bp in length, without sex-specific transtript. The open reading frame of tra-2 was 753 bp in length, which encoding a polypepide of 250 anino acids. tra -2 gene of B. cucurbitae had diferent expression levels in dfferent developmental stages. I1 had matermal genetic ffects with high expression in the embryonic period, and female adult expressed significantly more than male adult.