首页 | 本学科首页   官方微博 | 高级检索  
     


Induced spermiation of Pimelodus britskii (Teleostei: Pimelodidae) during the reproductive period
Authors:Danielle Zanerato Damasceno  Ricardo Andrei Krause  Maurício Spagnolo Adames  Giovano Neumann  Anderson Gibathe  Robie Allan Bombardelli  Elizabeth Romagosa
Affiliation:1. Centro de Aquicultura da Universidade Estadual Paulista “Júlio de Mesquita Filho”, Jaboticabal, SP, Brazil;2. Universidade Estadual do Oeste do Paraná, Toledo, PR, Brazil;3. TRACTEBEL Energia, UHE Salto Santiago, Saudade do Igua?u, PR, Brazil;4. Instituto de Pesca‐APTA, SAA, SP, Brazil
Abstract:For the first time was characterized the semen of Pimelodus britskii, hormonally induced and non‐induced induced, during the reproductive period. The experiment 1 was conducted with 12 fish per month, divided into: (i) induced with Carp Pituitary Extract (CPE), and (ii) without hormonal induction (testes macerated). The experiment 2 was conducted, with 30 fish, divided into groups for comparison of different doses of CPE and human Chorionic Gonadotrophin (hCG; T1: control; T2: 3.0 mg kg?1 CPE; T3: 0.5 mg kg + 3.0 mg kg?1 CPE; T4: 0.5 mg kg?1 + 7.0 mg kg?1 CPE; T5: 200 UI kg?1 hCG), the same fish were used every month, and the semen was collected by abdominal massage. In both, experiments were assessed the sperm motility and velocity by means of the CASA software. In experiment 1, the concentration of spermatozoids was significantly increase by application of CPE compared untreated males. The volume and motility decreased gradually until the end of the experiment, the highest values were recorded for treatment induced (0.49 ± 0.25 mL and 62.18, respectively). The same occurred to Gonadosomatic Index, showing the smallest value at the end of the reproductive period. The fish from experiment 2 released reduced volume of watery semen (0.1 mL). The values of sperm concentration, motility velocity decreased gradually throughout the months. For P. britskii, the reproductive period influenced the production and sperm quality. Despite small seminal volume released the dose of 7.5 mg kg?1 of CPE proved effective.
Keywords:seminal characteristics  reproductive cycle  carp pituitary extract  human chorionic gonadotropin  semen production  semen quality
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号