| 马铃薯不同基因型幼芽茎外植体的组织培养 |
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| 引用本文： | 张永成,张凤军.马铃薯不同基因型幼芽茎外植体的组织培养[J].农业科技通讯,2009(6):44-46. |
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| 作者姓名： | 张永成 张凤军 |
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| 作者单位： | 青海省农林科学院,青海西宁,810016 |
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| 基金项目： | 国家科技支撑-马铃薯育种，青海省重点科技攻关-马铃薯食品加工型型品种选育 |
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| 摘 要： | 利用青薯168、青薯5号和青薯8号三个品种幼芽带节茎段和节间组织为材料，研究了马铃薯的再生体系，结果表明，愈伤组织诱导率为72．92％～92．59％，愈伤组织分化率为30．68％～51．13％，每个外植体形成的小枝梢数为2．3～9．1个，绿色小植梢生根长成健壮的小植株，故认为MS＋NAA0．25mg／L＋BAP1．5mg／L＋GA37mg／L＋蔗糖30g／L＋琼脂7g／L为愈伤组织诱导培养基．MS＋BAP2．25mg／L＋GA37mg／L＋KT2．0mg／L＋NAA2．25mg／L＋蔗糖30g／L＋琼脂7g／L为分化培养基组成的再生体系是有效的，特别对青薯8号更为明显。
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| 关 键 词： | 马铃薯 幼芽茎外植体 愈伤组织诱导 小枝梢分化 |
Tissue culture of bud stem explants from three Qinghai genotypies of potato |
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| Authors: | Zhang Yong-cheng Zhang Feng-jun |
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| Institution: | (Qinghai Academy of Agriculture and Forestry Science, Xining, 810016,China ) |
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| Abstract: | The present article deals with in vitro induction callus and shoot regeneration of bud stem explants from three Qinghai cultivars: Qingshu168, Qingshu5 and Qingshu8. The results shown that frequency of callus induction were 72.92-92.59%, frequency of differentiation were 30.68-51.13%, number of shoot per explant were 2.3-9.1. The suitable induction medium was MS +NAAO.25mg/L+ BAP1.5mg/L+GA37mg/L+ Sucrose 30g/L+ agar 7g/L. The suitable differentiation medium was MS+ BAP2.25mg/L+ GA37mg/L+KT2.0mg/L+NAA2.25mg/L+ Sucrose 30g/L+agar7g/U |
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| Keywords: | potato bud stem explant callus induction shoot differentiation |
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