一种轮状病毒特异抗体ELISA检测方法

在实验室条件下，建立较为快速的轮状病毒抗体ELISA检测方法。利用组织培养的轮状病毒SA11株经初步的浓缩和纯化后，作为包被抗原包被酶标板。SDS-PAGE分析表明，经浓缩纯化后，轮状病毒的主要抗原均存在。将测试血清用SA11中和后，轮状病毒或其抗原免疫血清(肠黏液，粪便悬液)的OD490值比中和前有明显的下降，下降比率大于45％，而对照组血清的OD490值与中和前相比没有明显的变化，下降比率小于9％。表明用此ELISA体系检测轮状病毒抗体具有特异性。

An ELISA Method for Rotavirus Specific Antibody Detection

A rapid ELISA method for Rotavirus antibody detection was established under the common laboratory conditions. Cell-cultured Rotavirus SA11 was concentrated and partial purified and then used to coat the 96-well microtiter plates. The virus concentration was analyzed by SDS-PAGE to confirm the existence of most virus antigens. The tested sera, intestinal fluids, and fecal supernatant were neutralized by SA11. After neutralization, the OD_(490) values of the samples collected from rotavirus or its antigens immunized animals decreased significantly(>45% of the original specimens),while the OD_(490) values of the control samples did not change obviously ( <9% of the original specimens).The result demonstrated that the ELISA method was specific for rotavirus antibody detection.