| 一种轮状病毒特异抗体ELISA检测方法 |
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| 引用本文： | 刘馨,孙茂盛.一种轮状病毒特异抗体ELISA检测方法[J].动物医学进展,2005,26(3):96-98. |
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| 作者姓名： | 刘馨 孙茂盛 |
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| 作者单位： | 中国医学科学院中国协和医科大学医学生物学研究所,云南昆明,650118;中国医学科学院中国协和医科大学医学生物学研究所,云南昆明,650118 |
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| 摘 要： | 在实验室条件下，建立较为快速的轮状病毒抗体ELISA检测方法。利用组织培养的轮状病毒SA11株经初步的浓缩和纯化后，作为包被抗原包被酶标板。SDS-PAGE分析表明，经浓缩纯化后，轮状病毒的主要抗原均存在。将测试血清用SA11中和后，轮状病毒或其抗原免疫血清(肠黏液，粪便悬液)的OD490值比中和前有明显的下降，下降比率大于45％，而对照组血清的OD490值与中和前相比没有明显的变化，下降比率小于9％。表明用此ELISA体系检测轮状病毒抗体具有特异性。
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| 关 键 词： | 轮状病毒 酶联免疫吸附试验 中和实验 抗血清 |
| 文章编号： | 1007-5038(2005)03-0096-03 |
| 修稿时间： | 2004年8月25日 |
An ELISA Method for Rotavirus Specific Antibody Detection |
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| LIU Xin,SUN Mao-sheng.An ELISA Method for Rotavirus Specific Antibody Detection[J].Progress In Veterinary Medicine,2005,26(3):96-98. |
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| Authors: | LIU Xin SUN Mao-sheng |
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| Abstract: | A rapid ELISA method for Rotavirus antibody detection was established under the common laboratory conditions. Cell-cultured Rotavirus SA11 was concentrated and partial purified and then used to coat the 96-well microtiter plates. The virus concentration was analyzed by SDS-PAGE to confirm the existence of most virus antigens. The tested sera, intestinal fluids, and fecal supernatant were neutralized by SA11. After neutralization, the OD_(490) values of the samples collected from rotavirus or its antigens immunized animals decreased significantly(>45% of the original specimens),while the OD_(490) values of the control samples did not change obviously ( <9% of the original specimens).The result demonstrated that the ELISA method was specific for rotavirus antibody detection. |
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| Keywords: | Rotavirus ELISA neutralizing experiment antiserum |
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