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Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis |
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| Authors: | Saori MIURA Noriyuki HORIUCHI Kotaro MATSUMOTO Yoshiyasu KOBAYASHI Shin-ichiro KAWAZU Hisashi INOKUMA |
| Affiliation: | 1)Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan;2)Department of Basic Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080–8555, Japan;3)National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan |
| Abstract: | Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomeswas detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL)without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to3 EcoRI and HindIII fragments in these 2 atypical EBLcattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. Theprobe also hybridized to a large number of EcoRI andHindIII fragments in 5 cattle with persistent leukosis. These resultssuggest that the detection of monoclonal integration of BLV provirus into the host genomemay serve as a marker of monoclonal proliferation and malignancy in difficult to diagnoseEBL cattle. |
| Keywords: | atypical enzootic bovine leukosis diagnostic marker monoclonal integration |