Biotechnological production of highly soluble daidzein glycosides using Thermotoga maritima maltosyltransferase |
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| Authors: | Li Dan Park Jin-Hee Park Jong-Tae Park Cheon Seok Park Kwan-Hwa |
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| Affiliation: | National Research Laboratory for Functional Food Carbohydrate and Center for Agricultural Biomaterials, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, South Korea. |
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| Abstract: | The use of soybean isoflavones in food products is limited due to their low hydrophilicity. To enhance its solubility, the isoflavone daidzin was transglycosylated as a model compound using Thermotoga maritima maltosyltransferase (MTase). Four novel transglycosylation products of daidzin were identified by TLC and MALDI-TOF MS: daidzein 7-O-triglucoside, daidzein 7-O-pentaglucoside, daidzein 7-O-heptaglucoside, and daidzein 7-O-nonaglucoside. The major product, daidzein 7-O-triglucoside, was purified by C(18) and gel filtration chromatography, and its molecular structure was determined using UV, IR, MALDI-TOF MS, and NMR. The solubility of daidzein 7-O-triglucoside was 7.5 x 10(4) times that of daidzin, suggesting that the transglycosylation greatly enhanced its water solubility. |
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