D-Alanine-D-Alanine Ligase Gene (ddl) of Erwinia chrysanthemi Strain EC16 I. Isolation and Gene Dosage Effect on Pectate Lyase Synthesis |
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| Authors: | Pongphen JITAREERAT Hiroyuki MATSUMOTO Masahiro UMEHARA Shinji TSUYUMU |
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| Institution: | (1) The United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Shizuoka 422-8529, Japan, JP;(2) Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan, JP |
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| Abstract: | New regulatory gene for pectate lyase (Pel) production of Erwinia chrysanthemi strain EC16 was searched by observing the gene dosage effect of each cosmid library in Pel production. From this survey, a
cosmid clone, p5A, had reduced in Pel production under both inducing and non-inducing conditions and caused less tissue maceration
of potato tubers. The 2.64-kb HindIII fragment from p5A was found to be responsible for this phenotype and to contain one major ORF consisting of 1107 nucleotides,
which had homology with ddlA (49.6%) and ddlB (49.6%) of Escherichia coli and with ddlA (44.6%) of Salmonella typhimurium. These genes had been shown to encode D-alanine-D-alanine ligase (Ddl), an enzyme which is involved in the synthesis of peptidoglycan. This ORF encodes 368 amino acid residues
and its molecular weight is estimated to be 43 kDa. This ORF of EC16 could complement ddl deficient mutant of Escherichia coli.
Received 15 May 2002/ Accepted in revised form 20 June 2002 |
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| Keywords: | : : Erwinia chrysanthemi D-ala-D-ala ligase gene dosage effect pectate lyase maceration |
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