南美白对虾死后组织自溶规律的研究

为区别生前病变和死后变化,确定南美白对虾病理组织取材的有效时间,对南美白对虾组织自溶的规律进行了研究。分别于死后0,0.5,1,2,3 h和 4 h取材作常规组织学切片。在光学显微镜下观察,发现死后 2 h脑、触角腺以及肝胰腺的自溶较为明显,而其他器官在死后 4 h 仍未见明显的自溶现象。研究表明南美白对虾病理组织取材时间在死后2 h内为宜。

Study on the Law of Postmortem Autolyzation of Penaeus vannamei

In order to ensure the effective sample time of Penaeus vannamei and to distinguish postmortem changes from the pathologic changes occur before death.The samples of postmortem autolyzation on Penaeus vannamei.0,0.5,1, 2, 3 h and 4 h postmortem shrimps were, fixed and made into ordinary microslides. It was found that, brain, atennal gland and midgut gland (hepatopancreas) began to autolyze markedly at 2 h postmortem, while other organs did not show autolytic appearance even at 4 h postmortem. Thus the results indicated that the appropriate sampled time for Penaeus vannamei should be 0 h~2 h postmortem.