华南毛蕨挥发油对美洲斑潜蝇成虫的行为干扰作用

利用气相色谱一质谱连用仪，对华南毛蕨Cyclosorus parasiticus挥发油成分进行分析．结果表明，华南毛蕨挥发油中含有38种化学成分，酯类物质占21．961％，酸类占17．199％，烯醇类占16．161％，酚类物质占14．263％，醛酮类占11．026％，其中，对昆虫具有驱避作用的糠醛占2．943％，香豆素占1．327％，丁二酸二异丁酯占1．126％．对挥发油进行生物测定的试验结果表明，2．5、5.0、7．5、10．0μL用量对美洲斑潜蝇成虫产卵在施用24h有驱避作用同时表现出一定的拒食作用，其产卵驱避和拒食作用的大小随施用剂量的增加而增大，随时间的增长而逐渐减弱；施用48h后仅10．0μL有产卵驱避作用和拒食作用．采用四臂嗅觉仪对美洲斑潜蝇成虫的行为反应测定结果也表明，其对这种挥发油具有同样的行为趋向．

Deterrent effect of volatiles from Cyclosorus parasiticus on adult of Liriomyza sativae

Thirty-eight compounds, including furfural, coumarin and succinic acid, bis (2-methylpropyl)ester, were found in the volatiles extracted from Cyclosorus parasiticus, by using facility GC/MS. The bioassay showed that the volatiles in different concentration had the oviposition deterrent effect and antifeeding effect against adults of Liriomyza sativae in 24 h after treatment. Furthermore, the effect enhanced with the concentrations improving and weakened with time spending after treatment. The results of the four-armed olfactomater test showed that the volatiles from C. parasiticus influenced the beheivor of L. sativae strongly.