| 紫菜薹花青素合成酶基因BcANS的克隆、表达与序列分析 |
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| 引用本文： | 蒋明,陈孝赏,李金枝. 紫菜薹花青素合成酶基因BcANS的克隆、表达与序列分析[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4). DOI: 10.3785/j.issn.1008-9209.2011.04.006 |
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| 作者姓名： | 蒋明 陈孝赏 李金枝 |
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| 作者单位： | 1. 台州学院生命科学学院,浙江临海,317000
2. 台州市农业科学研究院,浙江临海,317000 |
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| 基金项目： | 浙江省自然科学基金资助项目，浙江省台州市科技计划资助项目 |
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| 摘 要： | 根据前期在芥蓝中克隆的BaANS基因序列设计PCR引物,从紫菜薹(Brassica campestris var.purpurea)子叶中克隆到基因组DNA和cDNA序列,基因定名为BcANS;序列已提交NCBI数据库,登录号为GQ120562；BcANS的基因组DNA全长为1 637 bp,具1个560 bp的内含子,编码区全长为1 077 bp,编码358个氨基酸.RT-PCR检测结果表明:BcA NS在光照条件下表达,在暗培养植株的子叶和胚轴中未见表达；缺磷植株的子叶和胚轴在暗培养和光照培养下BcANS基因均有表达,但光照条件下的表达量较大.序列比对结果表明:BcANS与同科的甘蓝、芥菜和拟南芥的同源性最高,而与禾本科植物的同源性最低,在进化树上相距最远.对紫菜薹花青素合成酶基因的克隆和表达分析,为进一步开展基因功能和花青素生物合成机制研究奠定了基础.
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| 关 键 词： | 紫菜薹 花青素合成酶 基因克隆 表达分析 |
Cloning, expression and sequence analysis of anthocyanidin synthase gene BcANS in Brassica campestris varpurpurea |
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| JIANG Ming,CHEN Xiao-shang,LI Jin-zhi. Cloning, expression and sequence analysis of anthocyanidin synthase gene BcANS in Brassica campestris varpurpurea[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2011, 37(4). DOI: 10.3785/j.issn.1008-9209.2011.04.006 |
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| Authors: | JIANG Ming CHEN Xiao-shang LI Jin-zhi |
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| Affiliation: | JIANG Ming1,CHEN Xiao-shang2,LI Jin-zhi1(1.College of Life Science,Taizhou University,Linhai,Zhejiang 317000,China,2.Taizhou Academy of Agricultural Sciences,China) |
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| Abstract: | PCR primers were designed according to BaANS gene isolated from Brassica albograbra,and the gene,designated BcANS,was amplified from cotyledons DNA and cDNA of B.campestris var.purpurea,respectively.The gene sequence was submitted to NCBI with an accession number of GQ120562.Genomic DNA was 1637 bp in length with one 560 bp intron,and the complete coding sequence was 1077 bp encoding 358 amino acids.RT-PCR results showed that BcANS expressed in cotyledons and hypocotyls under light condition,and there was n... |
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| Keywords: | Brassica campestris var.purpurea anthocyanidin synthase gene cloning expression analysis |
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