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| 均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺 | |
| 引用本文： | 宁颖,于海宁,周新妹,王维,蒋风,潘晓燕,沈生荣.均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺[J].浙江大学学报(农业与生命科学版),2011,37(3). |
| 作者姓名： | 宁颖 于海宁 周新妹 王维 蒋风 潘晓燕 沈生荣 |
| 作者单位： | 1. 浙江大学生物系统工程与食品科学学院,浙江杭州,310058 2. 浙江工业大学药学院,浙江杭州,310014 3. 嘉兴学院医学院,浙江嘉兴,314001 4. 杭州华东医药集团康润制药有限公司,浙江安吉,313301 |
| 摘 要： | 以SGC7901胃癌细胞存活率为指标,运用半仿生提取方法回收菱壳中的抗胃癌活性成分,考察提取时间、料液比、胃蛋白酶用量、胰蛋白酶用量对提取物抗胃癌活性的影响,并运用均匀设计法确定其最佳提取工艺.结果表明:菱壳与pH≈2提取液按料液质量体积比1∶10,不加入胃蛋白酶,37℃提取30 min后.离心收集沉淀,添加4%胰蛋白酶,菱壳与pH≈8提取液按料液质量体积比1∶10,37℃提取30 min,可获得最佳抑制效果,细胞存活率仅为45.52%.本实验表明,采用半仿生法提取菱壳中的抗胃癌活性成分是可行的. |
| 关 键 词： | 菱壳 抗胃癌活性 半仿生提取 均匀设计 |
Optimal process for semi-bionic extraction of bioactive compounds against gastric cancer from water chestnut shell by uniform designation |
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| NING Ying,YU Hai-ning,ZHOU Xin-mei,WANG Wei,JIANG Feng,PAN Xiao-yan,SHEN Sheng-rong.Optimal process for semi-bionic extraction of bioactive compounds against gastric cancer from water chestnut shell by uniform designation[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(3). | |
| Authors: | NING Ying YU Hai-ning ZHOU Xin-mei WANG Wei JIANG Feng PAN Xiao-yan SHEN Sheng-rong |
| Institution: | NING Ying1,YU Hai-ning2,ZHOU Xin-mei3,WANG Wei4,JIANG Feng4,PAN Xiao-yan3,SHEN Sheng-rong1(1.School of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China,2.College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,3.School of Medicine,Jiaxing College,Jiaxing,Zhejiang 314001,4.Hangzhou Huadong Medicine Group Kangrun Pharmaceutical Co.Ltd.,Anji,Zhejiang 313301,China) |
| Abstract: | |
| Keywords: | water chestnut shell anti-gastric cancer activity semi-bionic extraction uniform designation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |