Metabolic profiling of laser microdissected vascular bundles of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
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Authors: | Martina?Schad Rajsree?Mungur Oliver?Fiehn Email author" target="_blank">Julia?KehrEmail author |
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Institution: | (1) Department Lothar Willmitzer, Max-Planck-Institute of Molecular Plant Physiology, 14424 Potsdam, Germany;(2) UC Davis, 4321 GBSF Building Health Sciences Drive, Davis, CA 95616, USA |
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Abstract: | Background Laser microdissection is a useful tool for collecting tissue-specific samples or even single cells from animal and plant tissue
sections. This technique has been successfully employed to study cell type-specific expression at the RNA, and more recently
also at the protein level. However, metabolites were not amenable to analysis after laser microdissection, due to the procedures
routinely applied for sample preparation. Using standard tissue fixation and embedding protocols to prepare histological sections,
metabolites are either efficiently extracted by dehydrating solvents, or washed out by embedding agents. |
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