| 棉铃虫氨肽酶N基因片段克隆、表达和内源蛋白检测 |
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| 引用本文： | 刘京国,赵晓萌,杨爱珍,于同泉,路苹,师光禄.棉铃虫氨肽酶N基因片段克隆、表达和内源蛋白检测[J].北京农学院学报,2010,25(1):20-22,26. |
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| 作者姓名： | 刘京国 赵晓萌 杨爱珍 于同泉 路苹 师光禄 |
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| 作者单位： | 北京农学院生物技术系,北京,102206;北京农学院农业部都市农业(北方)重点实验室,北京,102206;北京农学院农业应用新技术北京市重点实验室,北京,102206 |
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| 基金项目： | 北京市自然科学基金(5092007);;北京农学院三项基金重点项目(08-ZD-SW-01);;北京市属市管高等学校人才强教计划资助项目 |
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| 摘 要： | 氨肽酶N（APN）是苏云金芽孢杆菌杀虫毒素Cry在昆虫中肠中的一个重要受体。研究氨肽酶N在昆虫中肠中的分布特征对于阐明Cry毒素的杀虫机理和昆虫对Cry毒素的抗性机理具有重要的意义。通过RT-PCR的方法从棉铃虫中肠上皮细胞中克隆得到氨肽酶N的基因片段APN1551,并诱导表达纯化得到其重组蛋白APN517。以此蛋白为抗原,制备其抗血清。用该抗血清能检测到棉铃虫中肠上皮细胞中的APN蛋白。为研究Cry毒素的作用机理奠定基础。
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| 关 键 词： | 氨肽酶N 棉铃虫 抗血清 |
Cloning, expression of aminopeptidase N (APN) from Helicoverpa armigera midgut and identification of endogenous APN |
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| LIU Jing-guo,ZHAO Xiao-meng,YANG Ai-zhen,YU Tong-quan,LU Ping,SHI Guang-lu.Cloning, expression of aminopeptidase N (APN) from Helicoverpa armigera midgut and identification of endogenous APN[J].Journal of Beijing Agricultural College,2010,25(1):20-22,26. |
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| Authors: | LIU Jing-guo ZHAO Xiao-meng YANG Ai-zhen YU Tong-quan LU Ping SHI Guang-lu |
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| Institution: | a,b(a.Department of Biotechnology;b.Key Laboratory of Urban Agriculture(North),Ministry of Agriculture;c.New Technological Key Laboratory of Agricultural Application in Beijing,Beijing 102206,China) |
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| Abstract: | Aminopeptidase N(APN) is one of important receptors in the insect midgut that Cry toxin from Bacillus thuringiensis(Bt) binds.To illuminate the mechanism of Cry toxin insecticidal action and inscet resistance to Cry toxin,it is necessary to study the distribution of APN in the insect midgut.In this paper,the APN gene fragment APN1551 by RT-PCR from Helicoverpa armigera midgut was cloned.This gene fragment was inserted into pGEX-4T-1 and the recombinant protein APN517 was induced and purified.Then the antise... |
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| Keywords: | aminopeptidase N Helicoverpa armigera antiserum |
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