鸡微卫星DNA标记与肉品质性状关系研究

利用25个微卫星标记，对以隐性白羽鸡和仙居鸡为亲本建立的资源群体F2500只鸡进行遗传检测，并测定各个体的鸡肉pH值、失水率、嫩度和肌间脂肪含量，采用方差分析法对标记与性状进行相关分析。结果表明：各位点平均等位基因数为5，平均杂合度为0．7012，平均多态信息含量为0．6468。嫩度间与pH值存在显著的正相关（P〈0．05），与肌间脂肪存在极显著的正相关（P〈0．01），与失水率之间存在显著负相关（P〈0．05）。方差分析显示：ADL136对肌间脂肪含量的影响极显著（P〈0．01）；MCW0095和ADL166对嫩度影响显著（P〈0．05）；MCW264对pH值影响显著（P〈0．05）；MCW264、ADL211和MCW0223对失水率影响极显著（P〈0．01）；其他标记对性状的影响未达到显著水平。

Effect of Microsatellite DNA Markers on Meat Quality Traits in Chickens

Twenty five microsatellite markers,selected from poultry genome were used to study genetic diversity and four meat quality traits on F_2 generation obtained from the mating of Recessive White chicken with Xianju chicken.Effects of 6 loci on meat quality traits were analyzed with PROC GLM of SAS 9.0 statistical software.The results indicated that,the averages of allelic number,heterozygosity(H) and polymorphism information content(PIC) were 5,0.701 2 and 0.646 8,respectively.There were positive correlations between tenderness and pH values(P<0.05) and high positive correlations between tenderness and IMF(P<0.01).On the other hand,the correlation between tenderness and water-losing rate was negative and of high values(P<0.01).The effect of ADL136 on IMF and MCW264,ADL211 and MCW0223 on water-losing rate were hightly significant(P<0.01),while the effect of MCW0095 and ADL166 on tenderness and MCW264 on pH values was also significant(P<0.05).No other significant effects on the four meat quality traits were obtained.