| 黑曲霉原生质体转化效率影响因子研究 |
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| 引用本文： | 王敬国,张卫国,夏林,邹德堂. 黑曲霉原生质体转化效率影响因子研究[J]. 东北农业大学学报, 2007, 38(4): 511-514 |
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| 作者姓名： | 王敬国 张卫国 夏林 邹德堂 |
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| 作者单位： | 东北农业大学学院,哈尔滨,150030 |
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| 摘 要： | 研究不同培养基、工具酶系统、洗涤液及加入抗性质粒混合次数对黑曲霉原生质体转化效率的影响。结果表明,优化条件后每10μg质粒转化子可达500个左右。
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| 关 键 词： | 黑曲霉 原生质体 转化效率 影响因子 |
| 文章编号： | 1005-9369（2007）04-0511-04 |
| 修稿时间： | 2006-01-05 |
Studies on effect factor of protoplast transformation frequency of Aspergillus niger |
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| WANG Jingguo,ZHANG Weiguo,XIA Lin,ZOU Detang. Studies on effect factor of protoplast transformation frequency of Aspergillus niger[J]. Journal of Northeast Agricultural University, 2007, 38(4): 511-514 |
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| Authors: | WANG Jingguo ZHANG Weiguo XIA Lin ZOU Detang |
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| Affiliation: | College of Agriculture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | This paper studied effects of medium composition,tool enzyme system,washing solution and number of times of mix when added the plasmid on Aspergillus niger protoplast transformation frequency.The result showed that the frequency obtained was 500 transformants per 10 μg plasmid after optimizing condition. |
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| Keywords: | A spergillus niger protoplasts transformation frequency effect factor |
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