| 山羊乳腺上皮细胞的光镜观察 |
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| 引用本文： | 郑月茂,安志兴,石玉强,张涌. 山羊乳腺上皮细胞的光镜观察[J]. 中国农学通报, 2005, 21(9): 4-6 |
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| 作者姓名： | 郑月茂 安志兴 石玉强 张涌 |
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| 作者单位： | 西北农林科技大学生物工程研究所,陕西,杨凌,712100 |
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| 基金项目： | 国家科技部863高技术研究发展规划资助项目“家畜体细胞克隆技术体系的建立和应用”（2001AA213081）. |
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| 摘 要： | 采用电穿孔转化法,将EGFP(增强型绿色荧光蛋白)基因转化体外培养的山羊乳腺上皮细胞。细胞穿孔转化7d后在荧光显微镜下观察。结果显示,EGFP基因成功地转入体外培养的山羊乳腺上皮细胞,EGFP基因获得表达,6种电穿孔参数的转化效果基本相同。
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| 关 键 词： | 山羊乳腺上皮细胞 电穿孔 EGFP基因 |
| 收稿时间： | 2005-03-17 |
| 修稿时间： | 2005-03-21 |
Observed on Goat Mammary Epithelial Cells by Light Microscope |
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| Zheng Yuemao,An Zhixing,Shi Yuqiang,Zhang Yong. Observed on Goat Mammary Epithelial Cells by Light Microscope[J]. Chinese Agricultural Science Bulletin, 2005, 21(9): 4-6 |
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| Authors: | Zheng Yuemao An Zhixing Shi Yuqiang Zhang Yong |
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| Abstract: | The present experiment aimed to transfect EGFP gene into goat mammary epithelial cells which culcured in vitro using electrotransfection. The cells were observed under fluoroscope when they were cultured over seven days after being transfected. The results were as follows: EGFP gene was successfully transfected into goat mammary epithelial cells and the expressing of EGFP gene were observed. The electrotransfection efficiency were same using six kinds of electrotransfection parameter. |
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| Keywords: | Goat mammary epithelial cells Electrotransfection EGFP gene |
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