Species relationships in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis |
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| Authors: | Akito Kaga Norihiko Tomooka Yoshinobu Egawa Kazuyoshi Hosaka Osamu Kamijima |
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| Institution: | (1) Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, 1-1 Rokko, Nada, 657 Kobe, Japan;(2) Department of Genetic Resources, National Institute of Agrobiological Resources, Kannondai, 305 Tsukuba, Ibaraki, Japan;(3) Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ohwashi, 305 Tsukuba, Ibaraki, Japan;(4) Experimental Farm, Kobe University, 1348 Uzurano, 675-21 Kasai, Hyogo, Japan;(5) Laboratory of Plant Breeding, Faculty of Agriculture, Kobe University, 1-1 Rokko, Nada, 657 Kobe, Japan |
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| Abstract: | Summary The genetic variation among 23 accessions of 5 species in the subgenus Ceratotropis, genus Vigna, were investigated by random amplified polymorphic DNA (RAPD) analysis. A total of 404 fragments amplified with 24 primers were scored and analyzed by cluster analysis. The accessions used were separated into two main groups with an average of 70% differences. Within the main groups, five subgroups were recognized, which are in complete agreement with taxonomic species. Wild forms were always grouped with their most closely related cultivated forms and they showed variation in each species. The largest intraspecific variation was found in V. radiata (mungbean), in which wild forms (V. radiata var. sublobata) were highly different from each other and from cultivated forms. V. angularis (adzuki bean) showed the least variation and thus, was probably differentiated in relatively recent times. |
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| Keywords: | Ceratotropis genetic relationships Vigna RAPD analysis |
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