| 利用SRAP分析东北地区甜菜品系遗传多样性 |
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| 引用本文： | 王茂芊,吴则东,陈丽,王华忠.利用SRAP分析东北地区甜菜品系遗传多样性[J].中国糖料,2010(2):4-8,11. |
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| 作者姓名： | 王茂芊 吴则东 陈丽 王华忠 |
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| 作者单位： | 1. 内蒙古农业大学,呼和浩特,010018
2. 黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨,150080;中国农业科学院,北方糖料作物资源与利用重点开放实验室/中国农业科学院甜菜研究所,哈尔滨,150080 |
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| 基金项目： | 国家科技支撑计划重点项目，高产优质专用甜菜育种技术研究及新品种选育，国家甜菜产业技术体系建设项目 |
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| 摘 要： | 采用SRAP分子标记方法对东北地区的100份甜菜材料进行了遗传多样性分析。利用4个表型差异显著的甜菜品系对SRAP的88对引物组合进行扩增,筛选出有效引物组合33对。SRAP的33对引物组合共产生694条扩增带,其中有424条多态性条带,多态性条带的比率平均为61.0%。按照UPGMA方法进行聚类分析,在遗传距离0.20处,将参试材料分为四大类群,分别为高产低糖低抗型、中产高糖高抗型、高产高糖高抗型、高产低糖抗丛根病型。聚类结果与生物学和经济学性状分类基本吻合,较好地显示了甜菜材料丰富的遗传多样性和遗传基础的差异性。遗传相似系数平均值大小为国外引进品种0.8642单胚品系0.7910多胚四倍体品系0.7497多胚二倍体品系0.7101。从聚类图来看,只有个别材料和外国品种聚类到一起,可能是由外国品种杂交改良而成,遗传基础相近。从SRAP扩增条带来看,外国材料只有8～12条,东北材料有16～28条之多,中外材料之间确实存在较大差异,东北材料中缺少丰产基因型,可能是基因组成比较复杂所致。
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| 关 键 词： | 甜菜品系 SRAP标记 遗传多样性 遗传基础 |
Genetic Diversity Analysis of Sugarbeet in the Northeast Region by SRAP Molecule Marker |
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| WANG Mao-qian,WU Ze-dong,CHEN Li,WANG Hua-zhong.Genetic Diversity Analysis of Sugarbeet in the Northeast Region by SRAP Molecule Marker[J].Sugar Crops of China,2010(2):4-8,11. |
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| Authors: | WANG Mao-qian WU Ze-dong CHEN Li WANG Hua-zhong |
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| Institution: | WANG Mao-qian1,WU Ze-dong2,3,CHEN Li2,WANG Hua-zhong2,3(1.Agricultural University of Inner Mongolia,Hohhot 010018,China,2.The Key Laboratory of Sugarbeet Genetics Breeding,Heilongjiang University,Harbin 150080,3.The Key Laboratory of North Sugar Crop Resource , Utilization,Chinese Academy of Agricultural Sciences / Sugarbeet Research Institute of Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | The genetic diversity of 100 sugarbeets in the Northeast of China was determined by SRAP molecule marker.88 couple primer combinations amplification of SRAP(sequence-related amplified polymorphism) were done using four different phenotype sugarbeet lines.33 couple efficient combinations were screened which displayed 694 amplified bands,424 diversity bands(mean rate as 61.0%).The experimental materials were classified into four groups: high yield and low sugar content with low resistantance,mid yield and hig... |
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| Keywords: | Sugarbeet SRAP molecule marker Genetic diversity Heredity basis |
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