| 大麦黄矮病毒(BYDV-GPV)的循回传播机理 |
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| 引用本文： | 吴云峰,李 毅,魏宁生.大麦黄矮病毒(BYDV-GPV)的循回传播机理[J].西北农林科技大学学报(社会科学版),1997,25(6):11-14. |
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| 作者姓名： | 吴云峰 李 毅 魏宁生 |
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| 作者单位： | 西北农业大学植保系，北京大学生命科学院，中国农业科学院植保所 |
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| 摘 要： | 对植物组织中蚜虫口针分泌的胶状唾液染色观察发现，蚜虫刺探时口针穿行在细胞间隙，仅在吸食时下颚口针进入细胞内。禾谷缢蚜饲毒１２ｈ时ＲＴ－ＰＣＲ方法在后肠、血淋巴液和附唾液腺中检测到ＧＰＶ；而非介体麦长管蚜在ＧＰＶ毒源上吸食２４ｈ时，仅在后肠组织中检测到ＧＰＶ；当继续取食４８ｈ时，在后肠及血淋巴液中也发现了ＧＰＶ，但仍无能力进入附唾液腺，说明蚜虫后肠和附唾液腺对病毒均有专化选择作用，禾谷缢管蚜传播ＢＹＤＶ－ＧＰＶ时存在吸食、获毒和传毒三个阶段。
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| 关 键 词： | 大麦黄矮病毒 麦蚜传播 RT－PCR检测 |
Mechanism of the Circulative Transmission of Barley Yellow Dwarf Letuovirus |
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| Wu Yunfeng,Li Yi,Wei Ningsheng,Cheng Zhuomin,Zhou Guanghe,DuZhijiang.Mechanism of the Circulative Transmission of Barley Yellow Dwarf Letuovirus[J].Journal of Northwest Sci-Tech Univ of Agr and,1997,25(6):11-14. |
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| Authors: | Wu Yunfeng Li Yi Wei Ningsheng Cheng Zhuomin Zhou Guanghe DuZhijiang |
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| Institution: | 3(1 Department of Plant Protection,Northwestern Agricultural University,Yangling,Shaanxi 712100)(2 College of Life Science,Beijing University,Beijing 100871)(3 Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094) |
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| Abstract: | When Rhopalosiphum padi and sitobion avenae feed on GPV-infected
oats, stylet mainly passes through intercellula rpath of tissue.Then a pair of maxillar only inserts phoem cell to feed sap by observation of saliva sheath stained with 0. 3%
Coomassie brilliant blue.R. padi requires GPV and GPV virions present in hindgut and
hemolymph by feeding on virus oats for 8h, and GPV virions exist in accessory salivary
gland (ASG) after feeding 12 to 24h, but only in hindgut and hemolymph of nonvector
S.avenae after feeding 48h by RT-PCR method. |
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| Keywords: | barley yellow dwarf letuovirus aphid transmission RT-PCR analysis |
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