| 豚鼠气单胞菌超声灭活PEG结合疫苗的制备及免疫效果 |
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| 引用本文： | 宋颖,关万春,易鹏,邓婕,潘长旺,常彦祥. 豚鼠气单胞菌超声灭活PEG结合疫苗的制备及免疫效果[J]. 浙江农业学报, 2010, 22(2): 0 |
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| 作者姓名： | 宋颖 关万春 易鹏 邓婕 潘长旺 常彦祥 |
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| 作者单位： | 温州医学院,浙江 温州 325035 |
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| 基金项目： | 温州市科技计划项目，温州医学院科学发展基金 |
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| 摘 要： | 以超声波瞬间高温、高压及剪切力作为灭活方式,用聚乙二醇(PEG)作为超声介质及疫苗载体,将豚鼠气单胞菌制成PEG结合疫苗。该疫苗与传统疫苗分别经腹腔注射免疫接种锦鲤成鱼,经检测血细胞吞噬百分率、吞噬指数、抗菌活力、抗体效价及免疫保护力等指标,评价疫苗效果。结果表明PEG6000作为结合剂制备的疫苗其各指标和绝对值全部高于其他试验组。PEG6000疫苗免疫保护力相比也最高,达到80%,高于传统灭活疫苗267个百分点,说明PEG6000作为结合剂制备的疫苗效果最好。
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| 关 键 词： | 豚鼠气单胞菌 超声灭活 PEG结合疫苗 免疫保护力 |
Study on preparation and immune efftect of PEG conjugate vaccine of ultrasonic inactived Aeromonas hydrophila |
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| SONG Ying,GUAN Wan-chun,YI Peng,DENG Jie,PAN Chang-wang,CHANG Yan-xiang. Study on preparation and immune efftect of PEG conjugate vaccine of ultrasonic inactived Aeromonas hydrophila[J]. Acta Agriculturae Zhejiangensis, 2010, 22(2): 0 |
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| Authors: | SONG Ying GUAN Wan-chun YI Peng DENG Jie PAN Chang-wang CHANG Yan-xiang |
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| Affiliation: | Wenzhou Medical College, Wenzhou 325035, China |
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| Abstract: | PEG conjugate vaccine of Aeromonas hydrophila was made by the methods of transient high-temperature,high press and shearing force of ultrasonic,using PEG as ultrasonic medium and vaccine vector.The PEG conjugate vaccine and the traditional vaccine were injected Cryprinuscarpiod intraperitioneally respectively.The percentage of phagocytosis,the index of phagocytosis,antibacterial activity,antibody titer and immune protective efficiency were investigated.The results showed that all the testing indexes of PEG ... |
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| Keywords: | Aeromonas hydrophila ultrasonic inactivation PEG conjugate vaccine immune protective efficiency |
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