| 水稻花粉管通道法导入高粱DNA的SSR分子验证 |
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| 引用本文： | 罗洪发,钟秉强,杨正林,李云峰,何光华. 水稻花粉管通道法导入高粱DNA的SSR分子验证[J]. 分子植物育种, 2004, 2(4): 501-505 |
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| 作者姓名： | 罗洪发 钟秉强 杨正林 李云峰 何光华 |
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| 作者单位： | 西南农业大学农学与生命科学学院,农业部生物技术与作物品质改良重点开放实验室,重庆,400716;贵州省毕节地区农业科学研究所,毕节,551700;西南农业大学农学与生命科学学院,农业部生物技术与作物品质改良重点开放实验室,重庆,400716 |
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| 基金项目： | 本项目由重庆"十五"良种创新工程和重庆市自然基金资助. |
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| 摘 要： | 从60对SSR引物中筛选出17对,对供体高粱、受体水稻及其外源。DNA导入后代稳定材料进行了分子验证,结果表明供体高粱与受体水稻间存在多态性、受体水稻与后代稳定材料间出现多态性,供体高粱与部分后代稳定材料间出现相同的扩增带,部分供体与受体共有的特征带在后代中消失、而有些后代出现了供体和受体均没有的扩增带。这表明供体高粱的DNA不同片段已整合到水稻品种中,获得了不同的变异材料,这些。DNA片段能在后代中稳定遗传。
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| 关 键 词： | 花粉管通道法 SSR分子标记 水稻 高粱DNA |
The SSR Molecular Evidence of Rice Transformation via Pollen Tube Pathway |
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| Luo Hongfa, Zhong Bingqiang Yang Zhenglin Li Yunfeng He GuanghuaCollege of Agronomy and Life Science,Key Lab of Biotechnology and Crop Quality Improvement,Ministry of Agriculture,South-West Agricultural University,Chongqing, Bijie Institute of Agricultural Science,Bijie. The SSR Molecular Evidence of Rice Transformation via Pollen Tube Pathway[J]. Molecular Plant Breeding, 2004, 2(4): 501-505 |
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| Authors: | Luo Hongfa Zhong Bingqiang Yang Zhenglin Li Yunfeng He GuanghuaCollege of Agronomy Life Science Key Lab of Biotechnology Crop Quality Improvement Ministry of Agriculture South-West Agricultural University Chongqing Bijie Institute of Agricultural Science Bijie |
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| Affiliation: | Luo Hongfa1,2 Zhong Bingqiang1 Yang Zhenglin1 Li Yunfeng1 He Guanghua1*1College of Agronomy and Life Science,Key Lab of Biotechnology and Crop Quality Improvement,Ministry of Agriculture,South-West Agricultural University,Chongqing,4007162 Bijie Institute of Agricultural Science,Bijie,551700 |
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| Abstract: | Sorghum genome DNA had been transfered into rice variety via pollen tube pathway. In this paper, the molecular verification was performed by SSR technique. The result shown seventeen of sixty pairs of primers can reveal polymorphisms among donor, receptor and their stable offspring. Same bandings were amplified between donor and some offspring. Some bandings appeared both donor and receptor, however disappeared offspring, at the same time several bandings could be found based on offspring but not donor or receptor. This suggested sorghum DNA fragment had been integrated into receptor rice, and led to stably inherit. |
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| Keywords: | Pollen tube pathway SSR molecular marker Rice(Oryza sativa L.) Sorghum DNA |
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