| 茎点菌属产松脂醇二葡萄糖苷的培养条件优化(简报) |
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| 引用本文： | 王 维, 师俊玲, 杨保伟. 茎点菌属产松脂醇二葡萄糖苷的培养条件优化(简报)[J]. 农业工程学报, 2008, 24(6): 287-290. |
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| 作者姓名： | 王 维 师俊玲 杨保伟 |
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| 作者单位： | 西北农林科技大学食品科学与工程学院，杨凌 712100;西北农林科技大学食品科学与工程学院，杨凌 712100;西北农林科技大学食品科学与工程学院，杨凌 712100 |
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| 基金项目： | 西北农林科技大学青年学术骨干支持计划 |
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| 摘 要： | 用响应面分析法对一株杜仲内生茎点霉(Phoma sp.)生产松脂醇二葡萄糖苷(PDG)的培养条件进行了优化，获得了PDG产量与各培养条件的回归模型，根据模型确定出PDG的最佳生产条件为：初始pH值8.4，摇床转速193 r/min，装液量100 mL，最佳条件下的PDG产量可达30 mg/L。最佳条件下试验所得值与模型预测值间的偏差小于5%。
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| 关 键 词： | 茎点菌属 松脂醇二葡萄糖苷 液体培养 培养条件 响应面分析 |
| 收稿时间： | 2007-08-08 |
| 修稿时间： | 2008-01-10 |
Optimization of conditions for production of pinoresinol diglucoside by a strain of Phoma sp. |
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| Optimization of conditions for production of pinoresinol diglucoside by a strain of Phoma sp.[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2008, 24(6): 287-290. |
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| Abstract: | Response surface analysis was used to get the optimal conditions for production of pinoresinol diglucoside (PDG) by a strain of Phoma sp. (SP-16F) in liquid cultivation. Initial pH value, rotation speed and media volume were investigated. A model showed good agreement with the experimental data of PDG production and cultivation conditions. By solving the model, the optimal conditions for PDG production were obtained at initial pH 8.37, rotation speed 193 r/min, volume of medium 100 mL, inoculation volume 10%, and temperature 28℃. Under the optimal conditions, the PDG production reached 30 mg/L. The variation between the practical data and predicted data was less than 5%. |
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| Keywords: | Phonma sp. Pinoresinol diglucoside liquid cultivation cultivation condition response surface analysis |
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