广西猪源禽Ⅰ型副粘病毒的分离与鉴定

[目的]了解广西猪源禽Ⅰ型副粘病毒的流行特征、毒力强弱及基因类型,为今后开展猪源副粘病毒的相关研究提供参考,也为有效防控副粘病毒感染奠定基础.[方法]采集疑似发病猪组织病料,通过鸡胚接种传代分离病毒后,分别进行血凝(HA)与血凝抑制(HI)试验、平均死亡时间(MDT)和脑内接种致死指数(ICPT)测定,然后运用RT-PCR对分离株进行鉴定及扩增部分F基因片段,并对扩增片段进行克隆测序及比对分析.[结果]从广西疑似发病猪组织中分离获得一株猪源禽Ⅰ型副粘病毒,其HA、HI效价均为26,鸡胚最小致死量为10-8/0.1mL,MDT为60 h,ICPI为1.45;分离株F基因112~117位裂解位点的氨基酸组成为R-R-Q-R-R-F,与NDV标准强毒株HER33、F48E9的裂解位点一致;同源性分析结果表明,分离株与标准强毒株F48E9、中等毒力代表毒株BeaudetteC的核苷酸同源性分别为86.5%和85.7%,其推导氨基酸同源性分别为90.1%和90.8%.[结论]猪源禽Ⅰ型副粘病毒分离株为强毒株,属于基因Ⅱ型,是传统型毒株,广西地区禽Ⅰ型副粘病毒宿主范围呈不断扩大趋势.

Isolation and identification of porcine origin avian paramyxovirus serotype 1 in Guangxi

【Objective】The present experiment was conducted to further understand the epidemic features， toxicity levels， and genotypes of porcine origin avian paramyxovirus serotype 1 in Guangxi in order to provide the relevant data for carrying out future researches on porcine paramyxovirus， and to provide references for the prevention and control of the disease. 【Method】The virus was isolated from suspected samples of pigs using chicken embryos inoculation. The characteristics of the virus were determined with hemagglutination （HA）， hemagglutination-inhibition （HI） test， mean death time of chicken embryos （MDT）， and intracerebral phthogenicity index （ICPI）. The virus strain was identified and F gene fragments were amplified using RT-PCR and sequenced. 【Result】The pig source avian paramyxovirus serotypeⅠisolate was isolated successfully from suspected samples of pigs. Its titer of HA and HI was 26 and chick embryo minimal lethal dose was 10-8/0.1 mL. The MDT and ICPI of this isolate were 60 h and 1.45， respectively. Amino acid composition was R-R-Q-R-R-F of F gene 112-117 splitting sites， which was in accordance with those of NDV standard virulent strain HER33 and F48E9. The nucleotide homologues were 86.5 and 85.7%， respectively， and the amino acid homologues were 90.1 and 90.8% after comparing the F gene of strain with standard virulent strain F48E9 and moderate virulence strain Beaudette C. 【Conclusion】The isolate was virulent strain belonging to A PMV-1 gene typeⅡand traditional strains. It signified that hosts of APMV-1 were spreading and expanding constantly in Guangxi area.