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蛇含委陵菜提取物对人工感染鸡白痢的治疗效果
引用本文:陶俊宇,胡庭俊,班丽萍. 蛇含委陵菜提取物对人工感染鸡白痢的治疗效果[J]. 南方农业学报, 2012, 43(9): 1391-1394. DOI: 10.3969/j:issn.2095-1191.2012.09.1391
作者姓名:陶俊宇  胡庭俊  班丽萍
作者单位:广西大学动物科学技术学院,南宁,530005
基金项目:广西自然科学基金重点项目(2010GXNSFD013022);广西研究生教育创新计划资助项目(GXUIIT32526)
摘    要:[目的]探讨蛇含委陵菜提取物对雏鸡白痢的治疗效果.[方法]采用不同提取方法(水煎法、水煎醇提法、微波提取法、乙酸乙酯提取法、乙醇分级提取法)对蛇含委陵菜进行提取,测定不同极性段提取物的最小抑菌浓度(MIC),筛选出最佳提取方法,然后以蛇含委陵菜水提物自拟高、中、低3个剂量组,经口灌服人工感染鸡白痢沙门氏菌的1日龄雏鸡,以诺氟沙星为药物对照,评价其对雏鸡白痢病的治疗效果.[结果]水煎法获得的蛇含委陵菜水提物对鸡白痢沙门氏菌和鸡大肠杆菌的抑制效果最佳,MIC分别为5.21和10.41mg/mL;在人工感染治疗试验中,雏鸡在感染鸡白痢沙门氏菌18h后,以每天经口灌服1次蛇含委陵菜水提物(0.75 mL/羽),连续3d,其治疗有效率达86.7%.[结论]蛇含委陵菜水提物对革兰氏阴性菌具有良好的抑制效果,安全低毒,原材料价廉易得,具有较高的研发价值.

关 键 词:蛇含委陵菜   提取物   鸡白痢   沙门氏菌   治疗效果

Therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken
TAO Jun-yu,HU Ting-jun,BAN Li-ping. Therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken[J]. Journal of Southern Agriculture, 2012, 43(9): 1391-1394. DOI: 10.3969/j:issn.2095-1191.2012.09.1391
Authors:TAO Jun-yu  HU Ting-jun  BAN Li-ping
Affiliation:(College of Animal Science and Technology,Guangxi University,Nanning 530005,China)
Abstract:[Objective]The present experiment was aimed to evaluate the therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken.[Method]Five extracting methods viz.,water decoction,water decoction combined with ethanol extracting,microwave extracting,ethyl acetate extracting and ethanol grading extracting were used to extract Potentilla kleiniana and minimal inhibitory concentration(MIC) in several extracts of different polarity zones were measured to screen out the best extraction method;subsequently,the Potentilla kleiniana extract with three different dosages(high,moderate,and low) were drenched to treat one-day-old chicks infected with artificial Salmonella pullorum,then the therapeutic effects were assessed using norfloxacin as the medical control.[Result]The water extract,obtained from water decoction,showed the most potent antimicrobial activity to Salmonella pullorum and Escherichia coli in chicken,and the MIC of Salmonella pullorum and Escherichia coli were 5.21 and 10.41 mg/mL,respectively.The optimal treatment effective rate of chicken reached 86.7% through drenching 0.75 mL/chicken once a day for 3 days consecutively after infecting Salmonella pullorum for 18 hours.[Conclusion]Safe,inexpensive,and low-toxic Potentilla kleiniana extract could effectively inhibit gram-negative bacterium;hence,Potentilla kleiniana extract is of high research and developmental value.
Keywords:Potentilla kleiniana  extract  pullorum disease  Salmonella pullorum  therapeutic effect
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