Aeromonas hydrophila B-haemolysin: purification and examination of its role in virulence in 0-group channel catfish, Ictalurus punctatus (Rafinesque) |
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| Authors: | R. L. THUNE MAUREEN C. JOHNSON T. E. GRAHAM R. L. AMBORSKI |
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| Affiliation: | Departments of Veterinary Science, Louisiana Agricultural Experiment Station and Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, U.S.A.;Department of Microbiology, Louisiana State University, Baton Rouge, U.S.A. |
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| Abstract: | Abstract. A single step procedure for purification of B-haemolysin from Aeromonas hydrophila is described which recovered 94% of the haemolytic activity with a sixfold increase in specific activity. Toxicity of haemolysin in 0-group channel catfish was 0–6 mg of purified protein per gram offish. Of six strains of A. hydrophila evaluated for differential toxin production and virulence in 0-group channel catfish, three B-haemolysin-deficient strains were virulent while three B-haemolysin-producing strains were avirulent. |
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