土壤中降解涕灭威菌株的分离鉴定及降解特性

为探明土壤微生物对涕灭威的降解能力,用富集培养法分离驯化土壤中涕灭威的优势降解菌,初步筛选出了对涕灭威具有较高降解能力的菌株TB26和100-8。经过生理生化鉴定和16S rDNA序列同源性分析,将菌株TB26初步鉴定为克雷伯杆菌属(Klebsiella sp.),菌株100-8初步鉴定为枯草芽孢杆菌属(Bacillus sp.)。TB26和100-8生长的最适碳源分别为麦芽糖、D-果糖,最适氮源分别为蛋白胨、脲。基础无机盐培养基和缺氮培养基对两种菌的生长情况及降解率的影响不同,外加氮源能够提高100-8的降解率,而缺氮培养基中TB26的降解率较高。

Isolation and Identification of Soil Micro-organisms Capable of DegradingAldicarb and its Characteristics of Degradation

For exploring the degradation ability of aldicarb by microorganisms,two strains of bacteria capable of degrading aldicarb,named as TB26 and 100-8,were isolated from soil by enrichment culture method.TB26 and 100-8 were identified as Klebsiella sp.and Bacillus sp.respectively by biochemical-physiological identification and sequence analysis of the 16S rDNA.The optimum carbon source for growth of TB26 and 100-8 were maltobiose and D-fructose respectively,the optimum nitrogen source were peptone and urea for growth of TB26 and 100-8 respectively.Basic culture medium and culture medium without nitrogen source have different effects on the growth of bacteria and degrading effect.Additional nitrogen source can improve the degrading effect of strain 100-8.It was found that the strain TB26 has higher degrading effect in the culture without nitrogen source.