Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM |
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Authors: | Rashid Jahwarhar Izuan Abdul Samat Noraini Mohtar Wan Yusoff Wan |
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Institution: | School of Bioscience and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600, Bangi, Malaysia. |
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Abstract: | Optimization of three parameters, temperature (25-35 degrees C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-15% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31 C) temperature and 5.5% (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL-1 were recorded to be closed to the predicted value (44.80 IU mL-1). Under the optimized SSF condition (31 degrees C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IU mL-1 compare to before optimized (30 degrees C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL-1. |
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