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| 家兔细胞核移植技术研究 | |
| 引用本文： | 常万存,韩建永,马世援,张红卫,陈忠科,Noboru Fujihar,王庆国,刘文浩. 家兔细胞核移植技术研究[J]. 西北农业学报, 2002, 11(1): 17-19 |
| 作者姓名： | 常万存 韩建永 马世援 张红卫 陈忠科 Noboru Fujihar 王庆国 刘文浩 |
| 作者单位： | 1. 农业部动物遗传改良生物技术重点开放实验室,山东省农业科学院生物技术研究中心,山东济南,250100 2. 山东大学生命科学学院,山东济南,250100 3. College,of,Agriculture,Kyushu,University,Japan |
| 摘 要： | 以家兔16－32细胞期胚胎卵裂为核供体，显微注射到去核卵母细胞周隙中，共制作完成229枚重组胚，2次电融合后率达到76．8％。重组胚与兔胚成胎成纤维细胞共同培养，卵裂率为65．7％（23／35），桑囊率达到22．9％（8／35），均显著高于（P＜0．01）微滴直接培养结果（43．3％、13／30；16．7％，5／30）。将111枚重组胚手术移植到9只同期发情的受体母兔输卵管中，2只妊娠，其中1只完成足月胚胎发育产出克隆仔兔2只。 |
| 关 键 词： | 细胞核移植 电融合 胚胎生物技术 家兔 |
| 文章编号： | 1004-1389(2002)01-0017-03 |
| 收稿时间： | 2001-11-20 |
| 修稿时间： | 2001-11-20 |
Nuclear Transfer in Rabbits |
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| CHANG Wan-cun,HAN Jian-yong,MA Shi-yuan,ZHANG Hong-wei,CHEN Zhong-ke,Noboru Fujihar,WANG Qing-guo and LIU Wen-hao. Nuclear Transfer in Rabbits[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2002, 11(1): 17-19 | |
| Authors: | CHANG Wan-cun HAN Jian-yong MA Shi-yuan ZHANG Hong-wei CHEN Zhong-ke Noboru Fujihar WANG Qing-guo LIU Wen-hao |
| Affiliation: | Key Laboratory of Animl and Plant Genetic Improving Biotechnology, Ministry or Agriculture, Research Center of Biotechnology, Agricultural Academy of Shandong Province, Jinan Shandong 250100, China;Key Laboratory of Animl and Plant Genetic Improving Biotechnology, Ministry or Agriculture, Research Center of Biotechnology, Agricultural Academy of Shandong Province, Jinan Shandong 250100, China;Key Laboratory of Animl and Plant Genetic Improving Biotechnology, Ministry or Agriculture, Research Center of Biotechnology, Agricultural Academy of Shandong Province, Jinan Shandong 250100, China;College of Life Science, Shandong University, Jinan Shandong 250100, China;College of Life Science, Shandong University, Jinan Shandong 250100, China;College of Agriculture, Kyushu Universiry, Japan;Key Laboratory of Animl and Plant Genetic Improving Biotechnology, Ministry or Agriculture, Research Center of Biotechnology, Agricultural Academy of Shandong Province, Jinan Shandong 250100, China;Key Laboratory of Animl and Plant Genetic Improving Biotechnology, Ministry or Agriculture, Research Center of Biotechnology, Agricultural Academy of Shandong Province, Jinan Shandong 250100, China |
| Abstract: | Blastomeres of rabbits from cell-16 to ce11-32 stage were used as the donors for muclear transfer in this study.The blastomere was injected to the preivit elline of enucleated oocyte by micromanipulation.As the result, the fusion rate was 76.8% after twice electric stimulus for the 229 recombined embryos.The cleavage rate (65.7%, 23/35) and the rate developed to morula and blastocysts (22.9%, 8/35) for recom bined embryos were significantly increased(P<0.01) by co-culture of fetal fibroblasts compared with droplet direct culture(43.3%, 13/30; 16.7%, 5/30) respectively.Nine estrus synchronized recipients were transplanted 111 reconstructed embryos into their oviducts, two recipients were in pregnancy and one successfully delivered two cloned of fspring after full period of pregnancy. |
| Keywords: | Nuclear transfer Electric fusion Embryo biotechnology Rabbits |
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