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| 上海地区番茄烟粉虱传双生病毒PCR检测 | |
| 引用本文： | 张永平,朱为民,崔辉梅.上海地区番茄烟粉虱传双生病毒PCR检测[J].上海农业学报,2007,23(4):100-101. |
| 作者姓名： | 张永平 朱为民 崔辉梅 |
| 作者单位： | 1. 石河子大学农学院,石河子,832000;上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 2. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 3. 石河子大学农学院,石河子,832000 |
| 基金项目： | 上海市科技兴农科技攻关项目 |
| 摘 要： | 番茄黄化曲叶病毒属于双生病毒,根据其基因序列设计引物COPR、AV494和COPR、COPL,进行PCR检测,结果发现:从感病的DNA中扩增出了356 bp和570 bp目的片段,而健康植株和抗原材料DNA中无此扩增带,表明上海地区番茄黄化曲叶病毒属于烟粉虱传双生病毒。 |
| 关 键 词： | 番茄烟粉虱传双生病毒 |
| 文章编号： | 1000-3924（2007）04-100-02 |
| 收稿时间： | 2006-11-13 |
| 修稿时间： | 2007-09-27 |
PCR detection of whitefly-transmitted geminiviruses from tomato in Shanghai area |
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| ZHANG Yong-ping,ZHU Wei-min,CUI Hui-mei.PCR detection of whitefly-transmitted geminiviruses from tomato in Shanghai area[J].Acta Agriculturae Shanghai,2007,23(4):100-101. | |
| Authors: | ZHANG Yong-ping ZHU Wei-min CUI Hui-mei |
| Abstract: | According to the gene sequence of tomato yellow leaf curl virus under geminiviridae,two pairs of primers of COPR\AV494 and COPR\COPL were designed, and the PCR detection was carried out.The results showed that the target fragments of about 356 bp and 570 bp could be amplified from the infected sample instead of from healthy and antigen samples,which confirmed that the tomato yellow leaf curl virus from Shanghai area came under geminiviridae. |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |