| 一种从多克隆抗体库快速分离高特异性抗体的方法 |
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| 引用本文： | 张霞,李杰,郭巍,李国勋. 一种从多克隆抗体库快速分离高特异性抗体的方法[J]. 华北农学报, 2008, 23(5) |
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| 作者姓名： | 张霞 李杰 郭巍 李国勋 |
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| 作者单位： | 河北农业大学,生命科学学院,河北省农作物病虫害生物防治工程技术中心,河北,保定,071000;河北省生物研究所,河北,石家庄,050051;河北农业大学,生命科学学院,河北省农作物病虫害生物防治工程技术中心,河北,保定,071000;河北农业大学,生命科学学院,河北省农作物病虫害生物防治工程技术中心,河北,保定,071000;青岛农业大学,山东,青岛,266109 |
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| 基金项目： | 国家重点基础研究发展计划("973"计划)，河北省自然科学基金项目 |
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| 摘 要： | 为克服传统方法制备单克隆抗体复杂而费时的弊病,研究介绍了一种从多克隆抗体库中快速分离高特异性抗体的方法;根据抗原抗体特异性结合的原理,以大肠杆菌系统表达的甜菜夜蛾围食膜蛋白SeP89为抗原,从多克隆抗体库中分离其特异性抗体;成功从甜菜夜蛾围食膜蛋白抗血清库中分离SeP89蛋白的特异性抗体;以快速分离围食膜蛋白SeP89抗体为例,研究建立了一种快速从多克隆抗体库中分离特异性抗体的方法.
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| 关 键 词： | 抗体 cDNA克隆 围食膜蛋白 抗血清 Western blot |
A Method of Rapid and Efficient Isolation of Highly Specific Antibodies from an Antiserum Against a Pool of Proteins |
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| ZHANG Xia,LI Jie,GUO Wei,LI Guo-xun. A Method of Rapid and Efficient Isolation of Highly Specific Antibodies from an Antiserum Against a Pool of Proteins[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(5) |
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| Authors: | ZHANG Xia LI Jie GUO Wei LI Guo-xun |
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| Affiliation: | ZHANG Xia1,2,LI Jie1,GUO Wei1,LI Guo-xun1,3 (1.College of Life Science,Agricultural University of Hebei,Biological Control Centre of Plant Pathogens , Plant Pests of Hebei Province,Baoding 071000,China,2.Biology Institute of Hebei,Shijiazhuang 050051,3.Qingdao Agricultural University,Qingdao 266109,China) |
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| Abstract: | The common method for monoclonal antibody preparation is complex and time-consuming.So a procedure which allowed rapid and efficient isolation of target protein from a library of antibodies was reported here.According to immunology protocol,specific antibodies to a peritrophic membrane protein,SeP89(which was expressed in Escherichia coli),was isolated from a polycolonal antiserum against a pool of proteins from the PM of Spodoptera exigua.We successfully isolated highly specific antibodies to SeP89 protein... |
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| Keywords: | Western blot |
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