| 禽流感病毒H5亚型及H7亚型基因的引物设计与多重RT-PCR扩增（英文） |
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| 作者单位： | ZHANG Wen-hui1，GUO Hua1，WANG Wei-li2，LIU Ming3，QIAN Ai-dong1 1.Jilin Agricultural University，Changchun 130118;2.Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China，Changchun 130062;3.National Key Laboratory of Veterinary Biotechnology，Harbin Veterinary Research Institute of CAAS，Harbin 150001 |
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| 摘 要： | [Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus(AIV);[Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank,and design primers(by Primer Premier 5.0)on high homologous region of these sequences,and then amplified by RT-PCR.[Result] The multiplex RT-PCR amplification,agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV.[Conclusion] It is feasible to rapidly diagnose AIV through this method.
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Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification |
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| ZHANG Wen-hui,GUO Hua,WANG Wei-li,LIU Ming,QIAN Ai-dong .Jilin Agricultural University,Changchun ,.Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun ,.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of CAAS,Harbin. Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2008, 0(1) |
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| Authors: | ZHANG Wen-hui GUO Hua WANG Wei-li LIU Ming QIAN Ai-dong .Jilin Agricultural University Changchun .Jilin Entry-Exit Inspection Quarantine Bureau of the People's Republic of China Changchun .National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute of CAAS Harbin |
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| Affiliation: | ZHANG Wen-hui1,GUO Hua1,WANG Wei-li2,LIU Ming3,QIAN Ai-dong1 1.Jilin Agricultural University,Changchun 130118,2.Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,3.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of CAAS,Harbin 150001 |
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| Abstract: | [Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus(AIV);[Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank,and design primers(by Primer Premier 5.0)on high homologous region of these sequences,and then amplified by RT-PCR.[Result] The multiplex RT-PCR amplification,agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV.[Conclusion] It is feasible to rapidly diagnose AIV through this method. |
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| Keywords: | Avian influenza virus Primer Premier 5.0 DNAStar Multiplex RT-PCR amplification |
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