Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora |
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| Authors: | Mariko Shoji Hidenori Sato Remi Nakagawa Ryo Funada Takafumi Kubo Shinjiro Ogita |
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| Institution: | (1) Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan;(2) Kyushu Regional Forest Office, Forestry Agency, The Ministry of Agriculture, Forestry and Fisheries of Japan, Kumamoto, Japan;(3) Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu Toyama, 939-0398, Japan |
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| Abstract: | The effect of an osmoticum, polyethylene glycol (PEG), on somatic embryo production was examined using embryogenic cells of
Pinus densiflora. In the basal medium containing 30 μM abscisic acid and 6% maltose, the quality of the embryos formed was poor even though
somatic embryos were produced. The addition of PEG with molecular weight of 4000 or 8000 significantly enhanced the development
of both the quality and quantity of somatic embryos. Furthermore, higher levels of a constant osmotic pressure with PEG 8000
in a range from about 300 to 450 mmol/kg could remarkably enhance the morphogenesis of somatic embryos and their number of
embryos produced. A higher stable osmotic pressure with an appropriate molecular weight of PEG is a key factor for the production
of good quality somatic embryos in P. densiflora. |
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| Keywords: | Maturation Osmotic pressure polyethylene glycol (PEG) Pinus densiflora Somatic embryogenesis |
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