Main Factors Affecting Cucumber (Cucumis sativus L.) Haploid Embryo Development and Haploid Plant Characteristics |
| |
Authors: | J. Przyborowski K. Nlemirowicz-Szgzytt |
| |
Affiliation: | Department of Genetics and Horticultural Plant Breeding, Warsaw Agriculture University, ul. Nowoursy-nowska 166, PL-02-766 Warszawa, Poland;Department of Plant Breeding and Seed Production, University of Agriculture and Technology, ul. PI. Lódzki 3, PL-10-724 Olstyn, Poland. |
| |
Abstract: | This report gives the first detailed analysis of cucumber haploid plants and the first evaluation of genotype and season effects on haploid embryo development. Female flowers of four F1 varieties and two inbred lines were pollinated with 300 Gy-treated pollen and three-week old embryos were cultured in vitro on E20A medium. The variety ‘Polan’ produced the greatest number of embryos (1.34 per 100 seeds), 51 % of which were capable of further development. The number of embryos able to develop was four times higher in summer than in spring. All the plants obtained, except one, were haploids or aneuhaploids. Haploid plants grew slowly and were sterile, although they occasionally set seedless fruits. The length of the stems, leaves and guard cells was smaller than those of diploids. |
| |
Keywords: | Cucumis sativus pollen irradiation embryo chromosome pollination in vitro culture sterility |
|
|