| 黄姜根茎组培快繁技术研究 |
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| 引用本文： | 易诚,张天晓,梁称福.黄姜根茎组培快繁技术研究[J].黑龙江农业科学,2008(1):11-13. |
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| 作者姓名： | 易诚 张天晓 梁称福 |
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| 作者单位： | 1. 湖南环境生物职业技术学院,衡阳,421005
2. 邵阳学院,邵阳,422000 |
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| 基金项目： | 湖南教育厅科技计划项目
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湖南省科技厅科技攻关项目 |
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| 摘 要： | 探索了黄姜离体培养快速繁殖的有效途径。结果表明：在附加6-BA的MS培养基上，外植体可诱导出大量的致密愈伤组织，而且致密愈伤组织在分化培养基中能分化产生大量圆球茎和幼苗；而在附加2，4-D的MS培养基中，外植体被诱导出疏松愈伤组织，在随后的分化培养基上只能产生数量有限的幼苗。带枝条的圆球茎在MS＋NAA0．5mg·L^-1＋IAA0．5mg·L^-1生根培养基上的生根率达到88．2％；移栽成活率迭85％。
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| 关 键 词： | 黄姜 组织培养 快速繁殖 |
| 文章编号： | 1002-2767（2008）01-0011-03 |
| 收稿时间： | 2007-06-06 |
| 修稿时间： | 2007年6月6日 |
Studies on Rhizome Culture and Rapid Propagation of Dioscorea Zingi berensis C. H. Wright |
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| YI Cheng,ZHANG Tian-xiao,LIANG Cheng-fu.Studies on Rhizome Culture and Rapid Propagation of Dioscorea Zingi berensis C. H. Wright[J].Heilongjiang Agricultural Science,2008(1):11-13. |
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| Authors: | YI Cheng ZHANG Tian-xiao LIANG Cheng-fu |
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| Institution: | YI Cheng, ZHANG Tian-xiao , LIANG Cheng-fu (1. Hunan Environment-biological Polytechnic, Hengyang 421005 ; 2. Shaoyang College, Shaoyang 422000) |
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| Abstract: | The effective approach of rhizome culture and rapid propagation of Dioscorea Zingi berensis C. H. Wright was probed into in this paper. The results showed that： a great quantity of compacted callus were induced on the MS medium supplemented with 6-BA and a large amounts of corms and young seedlings were differentiated on the differentiation medium, but plenty of loosened callus on the MS medium supplemented with 2,4-D and limited young seedlings on the differentiation medium. The rooting rate of the corms with branches amounted to 88. 2% on the medium of MS＋NAA0.5 mg· L^-1 ＋IAA0.5 mg·L^-1 and the transplanting survival rate reached 85 %. |
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| Keywords: | Dioscorea Zingi berensis C H Wright tissue culture rapid propagation |
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