猪源牛病毒性腹泻病毒的流行初探

根据牛病毒性腹泻病毒(BVDV)代表株NADL的NS5B基因序列,设计合成2对引物进行套式PCR,检测从浙江、安徽、湖南、江西、广西、辽宁等地采集的43份猪病料中BVDV的流行情况,结果有7份病料可以扩增出360bp的特异性条带,阳性率为16.3%。为追踪BVDV在猪体中的流行来源,我们又对细胞培养用国产及进口犊牛血清进行了检测,结果也能扩增到目的条带。对这些病料和血清中扩增出的目的条带进行克隆、测序,并用DNASTAR软件分析,结果表明这些目的片段均为BVDV序列,这些不同BVDV序列可以分为两个亚群,ZJ133、HN386、LN247、NCS-J属于BVDV-Ⅰa亚型,ZJ114、AH138、JX60、GX96、NCS-G、DZ属于BVDV-Ⅰb亚型。这些毒株与BVDVI型间的同源性为80.3%-98.6%,与BVDV-Ⅱ型的同源性为72.2%-74.4%。本文研究结果说明我国猪群中BVDV感染情况已经比较严重,应该予以重视。

The Initial Study on the Prevalence of Bovine Viral Diarrhea Virus(BVDV) from Swine

Based on the published NS5B region sequence of bovine viral diarrhea virus (BVDV) strain NADL, two sets of BVDV specific primers were designed for detection of BVDV by nested polymerase chain reaction(RT-PCR) in 43 swine tissue samples from Zhejiang , Anhui, Hunan, Jiangxi, Guangxi and Liaoning province. The results showed that positive rate was 16.3% by amplifying special fragments of 360bp from 7 tissue samples. In order to find the infection source, external and domestic serums of cell culture were also extracted and amplified,and positive bands of BVDV were obtained. The expected fragments were cloned, sequenced and analyzed by DNAstar software. It was shown that all of positive fragments are BVDV sequences which can be divided into two subgroups: ZJ133、HN386、LN247、NCS-J belong to BVDV -Ⅰa subtype while ZJ114、AH138、JX60、GX96、NCS-G、 DZ belong to BVDV-Ⅰb subtype. Compared to the type BVDV-Ⅰand Ⅱ, these strains has the homology between 80.3%-98.6% and 72.2%-74.4%.It is concluded that the BVDV infection in swine has been serious in the country.