| 三角褐指藻细胞破碎方法的研究 |
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| 引用本文： | 汤卫华,岳鹍,王芃,殷海松.三角褐指藻细胞破碎方法的研究[J].农产品加工．学刊,2012(2):48-49,69. |
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| 作者姓名： | 汤卫华 岳鹍 王芃 殷海松 |
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| 作者单位： | 天津现代职业技术学院生物化工系 |
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| 摘 要： | 三角褐指藻是一种含有较高多不饱和脂肪酸的海洋单胞藻。为了从细胞中提取不饱和脂肪酸,采用反复冻融法、超声波破碎法和匀浆法分别对三角褐指藻细胞进行破碎,并测定油脂提取率。结果表明,超声波破碎法、反复冻融法和匀浆法的最大细胞破碎率分别为91.5%,56.7%和81.2%;油脂提取率分别为18.1%,13%和13.4%。从操作的简捷性和经济效益来看,超声波破碎法优于反复冻融法和匀浆法。
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| 关 键 词： | 三角褐指藻 反复冻融法 超声波破碎法 匀浆法 油脂 |
Research on the Methods of Phaeodactylum tricornutum Cells Fragmentation |
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| TANG Wei-hua,YUE Kun,WANG Peng,YIN Hai-song.Research on the Methods of Phaeodactylum tricornutum Cells Fragmentation[J].Nongchanpin Jlagong.Xuekan,2012(2):48-49,69. |
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| Authors: | TANG Wei-hua YUE Kun WANG Peng YIN Hai-song |
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| Institution: | (Department of Chemical Biology,Tianjin Modern Vocational Technology College,Tianjin 300350 China) |
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| Abstract: | The marine diatom Phaeodactylum tricornutum is rich in polyunsaturated fatty acids(PUFAs).In order to extract PUFAs from cells,three methods of cells fragmentation,including ultrasonic method,freezing-thawing method and homogenate method are introduced.The result shows that cell disruption rates are 91.5%,56.7% and 81.2% respectively; extraction rates are 18.1%,13% and 13.4%.Considering the operating convenience and economic benefits,the ultrasonic method is superior to the methods of freezing-thawing and homogenate. |
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| Keywords: | Phaeodactylum tricornutum freezing-thawing method ultrasonic method homogenate method total lipid |
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